Protocols

Metabolite Assays:

Leaf Fatty Acid Methyl Esters (FAMEs)

Leaf Amino Acid Assay (and the HLPC-MS/MS method that we use)

Seed Amino Acid Assay (and the HLPC-MS/MS method that we use)

Seed Carbon/Nitrogen Ratio

Leaf Starch Assay

Seed Starch Assay

Morphology and Physiology Assays:

Chloroplast Morphology Assay (and examples of images that correspond to different controlled vocabulary terms)

Seed Morphology Assay (and examples of seeds that correspond to different controlled vocabulary terms)

Chlorophyll Fluorescence Kinetics Assay

Whole Plant Morphology:

• How we photograph our plants
• How we set up for photography
• How we choose controlled vocabulary descriptions of morphology
• The template used for rapidly assessing rosette size

Genotyping Assay